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Informasi Alat
| Nama Alat | FACSLyric |
|---|---|
| Deskripsi Alat | FACSLyric 3L10C Instrument IVD Merek: BD Biosciences Optics Available system configurations 4-color: 2-laser (blue, red) (3-1) 6-color: 2-laser (blue, red) (4-2) 8-color: 3-laser (blue, red, violet) (4-2-2) 10-color: 3-laser (blue, red, violet) (4-3-3) 12-color: 3-laser (blue, red, violet) (4-3-5) Solid-state laser specifications Blue laser: 488 nm, 20 mw Red laser: 640 nm, 40 mw Violet laser: 405 nm, 40 mw Beam spot size (all lasers) 9 m x 63 m Optical alignment Auto alignment on demand Flow-cell lens 1.2 NA FSC detector Photodiode SSC and FL detectors PMT See filter guide for optical configurations. Fluidics Flow cell Stainless steel with low coefficient of thermal expansion for predictable, stable performance Cuvette internal cross-section 430 m x 180 m Sample flow rates Low: 12 L/min Medium: 60 L/min High: 120 L/min High sensitivity: 50 L/min Fluid capacity Standard 5-L tanks Optional 10-L tanks Adapter available for 20-L BD FACSFlowTM cubitainer Sheath core stream fluid velocity Normal: 5.4 m/s High sensitivity: 2.7 m/s Sheath fluid consumption Normal: 13.6 mL/min High sensitivity: 6.6 mL/min Supported tubes, plates and tube racks • With BD FACS Universal Loader Tubes 30-tube rack (12 x 75-mm tubes) 40-tube rack (12 x 75-mm tubes) Plates 96 Falcon® standard height, round, polystyrene 96 Falcon® standard height, flat, polystyrene 96 Falcon® standard height, round, polypropylene 96 Falcon® standard height, conical, polypropylene 384 Greiner standard height, flat, polystyrene 96 Falcon®, half deep, conical, polypropylene 96 Falcon®, deep, conical, polypropylene 96 Milipore, filter bottom, polypropylene • With manual tube port Falcon® 5 mL (12 x 75-mm) polystyrene and polypropylene BD TrucountTM 5 mL (12 x 75 mm) Falcon 15 mL Falcon 50 mL Microcentrifuge 2 mL Sample dead volume 30 μL (12 x 75-mm tubes) Cytometer schedule settings Pre-programmed startup and idle shutdown Software • Integrated bi-directional LIS interface using BD FACSLinkTM software • Support for 21 CFR Part 11 workflow with audit trail and e-signature • Universal setup for fast and convenient instrument setup and standardization • Single-tube QC with BDTM CS&T beads • QC module with Levey-Jennings plots • Two applications BD FACSuite Application • User-defined assays • User-defined plots • User-defined worksheets and reports • User-defined tube/reference settings • Expression editing BD FACSuite Clinical Application Pre-configured workflow and pre-set templates for the following BD IVD assays: • BD TritestTM • BD MultitestTM 4-Color BD Multitest 6-Color TBNK • BD TrucountTM control • Report in 26 languages QC Automated single-tube QC with BDTM CS&T beads Performance Acquisition rate Up to 35,000 events per second. No limit on number of events acquired in a single FCS file Carryover <0.10% with default SIT flush <0.05% with 3 or more SIT flushes Sensitivity FITC: <85 MESF PE: <20 MESF Channel Qr* (x1,000) FITC 20 PE 133 PerCP-CyTM5.5 13 PE-CyTM7 17 APC 10 BD HorizonTM APC-R700 8 APC-CyTM7 7 BD Horizon V450 47 BD Horizon V500 17 BD Horizon BV605 133 BD Horizon BV711 43 BD Horizon BV786 16 Fluorescence precision <3% CV for chicken erythrocyte nuclei (CEN) Fluorescence linearity 2 ±0.05% for CEN Data resolution Uncompensated data has a range of 0–262,143 SSC and FSC resolution Enables separation of 0.2-μm beads from noise System throughput 50 minutes for a 40-tube rack with a standard BD Tritest assay stopping rule on samples with normal CD4 counts (approximately 1190 cells/μl). 40 minutes for a 96-well plate, using default mix settings, a two-second asquisition, and a SIT flush in between each well and no preview before acquring or report review delay. Parameters Area (A), Width (W), Height (H) for all channels and Time (T). Total of 43 parameters available. 3 Scales: Linear (A, W, H), Logarithmic (A, H) Biexponential (A, W, H) Compensation Full inter-beam matrix, during or post acquisition Threshold Any single parameter or logical combination of multiple parameters Data management Workstation specifications (minimum required) Clock speed of 3.2 GHz 16 GB RAM Hard drive and data storage 1 TB Solid State HD Operating system Microsoft® Windows® 10 IoT 64-bit OS Peripheral devices At least 3 USB ports HP USB Keyboard US HP USB Optical Mouse Networking Ethernet LAN 10/100/1000 Signal Processing 18-bit dynamic range with IEEE 32 bit floating-point resolution Monitor LCD flat panel, 23 in. LCD flat panel, 29 in. (recommended) Data management options BD FACSLinkTM software for LIS connectivity. BD Assurity LincTM software for remote diagnostic capability Installation requirements Operating temperature 15°C (59°F) to 30°C (86°F) Maximum of ±2.5°C/day fluctuation recommended Humidity 15% to 85% relative humidity (noncondensing) Dimensions (W x D x H) Cytometer 63.2 x 57.9 x 57.9 cm 24.9 x 22.8 x 22.8 in. With standard tanks 85.2 x 57.9 x 57.9 cm 33.5 x 22.8 x 22.8 in. With standard tanks and loader 107.2 x 57.9 x 57.9 cm 42.2 x 22.8 x 22.8 in. Weight Cytometer: 56.0 kg (123.5 lb) Loader: 13.2 kg (29 lb) Power specifications Voltage: 100–240 ±10% VAC Frequency: 50–60 ±10% Hz Current: 2 A Power: 200 W Operational heat dissipation 488 BTU/hour at ambient temperature Noise under normal operating conditions 55 dBA over 8 hours under normal operating conditions Altitude 0.8 atm (approximately 2,000 meters) System options BD FACSTM Universal Loader Compatible with 30 (barcoded) or 40 (non-barcoded) tubes (12 x 75 mm). Equipped with an orbital shaker for in- place mixing and resuspension of cells. Optimized for all supported plate and tube formats. Includes internal barcode reader for positive sample identification. Supported barcode formats Codabar Code 128 Code 3 of 9 Interleaved 2 of 5 Handheld barcode scanner Handheld barcode scanner with stand supporting common 1-D and 2-D formats Extended-use fluidics Optional tanks and connectors to allow for use with 10-L waste tanks and BD FACSFlowTM cubitainers |
| Preparasi Sample | |
| Lokasi | Lab Doping lt 2 (SF) |
| PIC | Dr. Nova Suliska, S.Farm., M.Si. |
| Status | Active : Penggunaan Alat dapat dilakukan oleh semua users. |
| Waktu Penggunaan Alat | Per-jam |
